|Description:||Antibacterial Drug Target ׀ Type II DNA topoisomerases including topoisomerase II and topoisomerase IV hydrolyze ATP as the source of molecular energy to carry out DNA supercoiling and DNA decatenation reactions. Inhibition of the ATPase activity of topoisomerases by compounds such as novobiocin blocks their biological functions. Therefore, the topoisomerase ATPase assay can be used for high-throughput screen of topoisomerase inhibitors. The Topoisomerase ATPase Assay Kit is based on detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. The assay is in a 384-well plate format and the phosphate is detected using light absorbance at 650 nm. This product includes 400 ul of 10 x Buffer, 35 ul of 100 x DNA, 33 ul of 100 x P. aeruginosa Topo IV, 35 ul of 100 x ATP and 5 ml of Dye for 100 assays of P. aeruginosa Topo IV ATPase activity in a 384-well assay format.|
|Unit Size:||100 assays|
|Shipping:||Blue Ice (-20°C)|
|More information:||Go to webpage|
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