|Cyagen OriCell™ KO-certified 3R MEF are derived from 13.5-day old mouse embryos and have been genetically engineered to carry 3 genes that confer neomycin, hygromycin, and puromycin resistance. The cells are grown as a monolayer and cryopreserved following mitotic-arrest with γ-irradiation at first passage (P1). MEF cells are widely used as a feeder substrate for supporting the growth and maintenance of both mouse and human Embryonic Stem Cells (ESCs) in cell culture. Each lot of OriCell™ KO-certified 3R MEF has been tested to effectively maintain pluripotency and the undifferentiated state of human and mouse ESCs. In addition, OriCell™ KO-certified 3R MEF are used as a feeder substrate for ESCs used in Cyagen gene targeting projects, leading to successful generation of knockout and knockin animals. Additionally, the cells have greater than 80% post-cryopreservation viability and are free of bacteria, fungi, mycoplasma and endotoxin contamination.
|Only available in Germany and selected European countries.
|See manual & corresponding webpage
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