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|Terminal azide bearing nucleotides closely resemble natural nucleotides and are the basis of chemoenzymatic mRNA labeling. Due to the small size of the functional groups of nucleotides they are well accepted by T7 RNA polymerase and poly(A) polymerase and terminal deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA with baseclick approach is really modular and not limited by enzymatic compatibility of bulky pre-labeled nucleotides. Application: Enzymatic incorporation into DNA by PCR to produce ethynyl-functionalized DNA
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