|ExoFast™ Exosome Isolation Reagent from serum enables fast and efficient enrichment of intact exosomes from blood serum samples. Isolation of exosomes can be a tedious, non-specific, and difficult process. ExoFast™ Exosome Isolation Reagent from serum enables simple and reliable concentration of intact exosomes from blood serum samples with a protocol that is scalable depending on your sample size. By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the serum sample, and the solution is incubated at 2°C to 8°C for 30 min. The precipitated exosomes are recovered by standard centrifugation at 10,000 x g for 10 min. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods.
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