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Sensolyte® Cell Cytotoxicity Assay Kit

Sensolyte® Cell Cytotoxicity Assay Kit
Description: The damage of cell membrane leads to the release of cytoplasmicEnzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity. The SensoLyte® Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but it only measures the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need for extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of cytotoxicity of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. The kit contains:• Assay mixture • Assay buffer • Lysis solution• Stop solution • A detailed protocol Kit Size: 500 assays
Order #: AS-71302
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Blue Ice
Storage: -20°C
Subcategory: Fluorescent Dyes
More information: Go to webpage
Datasheet
275.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

    How To Order

    Orders can be placed by phone, fax, e-mail, or via our online shop:

    After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.

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