|Description:||Fluorescein di-β-D-galactopyranoside (FDG) is one of the most sensitive fluorogenic substrates available for detecting β-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties (Ex/Em=492/520 nm) that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect β?galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In general, fluorescence-based measurements can be several orders of magnitude more sensitive than absorption-based measurements. FDG has been widely used for identifying lacZ-positive cells with fluorescence microscopy and flow cytometry. FDG is also used to detect β-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.|
|Unit Size:||5 mg|
|Restrictions:||Only available in Germany and selected European countries.|
|Storage:||- 20 °C Darkness|
|Subcategory:||Labeling & Detection|
|More information:||Go to webpage|
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 (0)551 707 22 70
- E-mail: firstname.lastname@example.org
How To Order
Orders can be placed by phone, fax, e-mail, or via our online shop:
After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.