|Description:||Reactive oxygen species assay kit uses a unique fluorescent probe H2DCFDA to detect reactive oxygen species. H2DCFDA is chemically reduced and acetylated form of 2′,7′-dichloro- fluorescein (DCF) and is nonfluorescent and cell-permeant. Once H2DCFDA enters into cells, the acetate groups are removed by intracellular esterases to form H2DCF. H2DCF is well retained inside cells. Oxidation of H2DCF by reactive oxygen species yields fluorescent DCF. Reactive oxygen species can be detected by monitoring the increase in fluorescence with a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein (FITC). Because the dyes are susceptible to photo-oxidation, low light conditions should be used for fluorescence microscopy applications whenever possible.|
|Unit Size:||1000 assays|
|Subcategory:||Oxidative Stress Detection|
|More information:||Go to webpage|
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 days
- Phone: +49 (0)551 707 22 70
- E-mail: firstname.lastname@example.org
How To Order
Orders can be placed by phone, fax, e-mail, or via our online shop:
After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.