Description: | TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) involves an energy transfer between two fluorophores in close proximity: a donor molecule with long-lived fluorescence, such as terbium (Tb) and europium (Eu) and an acceptor molecule with short-lived fluorescence. TR-FRET pairs are chosen to achieve overlap of the donor emission and the acceptor absorbance spectra. Europium is usually paired with red-shifted dyes (allophycocyanine, either Cy5®, or CyLyte, or Alexa Fluor® 647) as energy acceptors. AnaTag™ Protein Labeling Kit for TR-FRET provides Europium (Eu) dye for generating Eu-protein conjugates, and acceptor red dye HiLyte™ Fluor 647. When Eu conjugates are used in TR-FRET with H647-labeled reagents the signal can be detected at excitation/emission wavelengths = 325 nm/667 nm. Kit Size: 1 conjugation reaction with Eu and 1 conjugation reaction with HiLyte Fluor™ 647. Each conjugation reaction can label up to 200 ug proteins (IgG). |
Order #: | AS-72247 |
Unit Size: | 1 kit |
Supplier: | AnaSpec |
Restrictions: | Only available in Germany and selected European countries. |
Shipping: | 4 °C |
Storage: | 4 °C |
Subcategory: | Labeling & Detection |
More information: | Go to webpage |
1,015.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
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