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Lentivirus Transduction Enhancer II

Lentivirus Transduction Enhancer II
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Description: ABP Biosciences offers three Lentivirus Transduction Enhancers.  Lentivirus Transduction Enhancer I is a novel chemical reagent that increases lentiviral transduction efficiency into in vitro systems. Normally, incubation of lentivirus particles in cells in the presence of polybrene is generally efficient in transducing many cell types. However, lentiviruses are typically attenuated by cellular antiviral defense mechanism, which limits their transducing efficiency. Lentivirus Transduction Enhancer I suppresses the cellular anti-viral state by mimicking the activity of viral virulence gene products, and thus significantly increases lentivirus transducing efficiency by as high as 10-fold. Lentivirus Transduction Enhancer II is a nontoxic novel chemical reagent that increases lentiviral transduction efficiency by modulating membrane permeability. Normally, incubation of lentivirus particles in cells in the presence of polybrene is generally efficient in transducing many cell types. However, due to polycation property, polybrene is toxic for some sensitive cells. Lentivirus Transduction Enhancer II is a neutral and nontoxic transduction enhancer, which significantly increases lentivirus transducing efficiency while maintaining cell membrane integrity. Polybrene As a cationic polymer, polybrene increases the efficiency of infection in some cells by neutralizing the charge repulsion between virions and the cell surface. Polybrene is most effective when added to cell culture media at the time of transduction. Recommended working concentration is 1:100. However, the working concentration is highly cell line-dependent. Lower or higher dilution ratio may be required to optimize the effect.
Order #: D024-ABP
Unit Size: 1 mL
Supplier: ABP Biosciences
Shipping: RT
Storage: RT
Subcategory: Lentivirus
More information: Go to webpage
Datasheet
133.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 days

    How To Order

    Orders can be placed by phone, fax, e-mail, or via our online shop:

    After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.

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