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|Description:||Transcreener cGAMP cGAS Assay. The Transcreener cGAMP cGAS Assay directly measures cyclic GMP-AMP (cGAMP) produced by cyclic GMP-AMP synthetase (cGAS, C6orf150, 2'3'-cGAMP Synthetase). These cGAMP measurements allow researchers to effectively determine the enzyme's activity. The assay provides a powerful tool to screen compound libraries for cGAS modulators to help find new therapies for disease. Important Messenger in the STING Pathway. DNA in the cytosol of eukaryotic cells indicates infection or cellular damage, and it elicits a robust immune response, driven by type I interferon (IFN) induction. cGAS is an exquisitely sensitive sensor for double-stranded DNA in the cytoplasm. Binding of DNA activates the enzyme to produce a unique cyclic dinucleotide second messenger, cGAMP, which acts as an agonist for the STING (stimulator of interferon genes) receptor, leading to induction of a type I interferon response. How Does This cGAS Assay Work? The Transcreener cGAMP cGAS Assay determines cGAS enzyme activity by directly measuring the cGAMP formed by the enzyme using the Transcreener cGAMP Assay. By detecting cGAMP output, the assay provides a universal method to assess the activity of any cGAMP-producing enzyme in real-time. Transcreener cGAMP Assay technology uses a simple but highly effective method that consists of an antibody selective to cGAMP and a far-red fluorescent tracer. cGAMP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout. The cGAS assay is available with FP and TR-FRET. It is a simple mix-and-read format. Perform your enzyme reaction, add the detection reagent, and measure. The simplicity of the system yields robust results that also makes it extremely amiable to HTS. The Role of cGAS As a Therapeutic Target. cGAS recognizes foreign double-stranded DNA and initiates an innate immune response, called the cGAS-STING pathway. cGAS can cause tissue or autoimmune diseases if overactivated by the body's abnormal DNA. Examples of diseases caused by this include Aicardi-Goutières syndrome, systemic lupus erythematosus, and primary biliary liver disease (Li et al, 2021). The cGAS-STING pathway is an attractive target for autoimmune diseases and cancers. Transcreener's cGAMP cGAS Assay is an excellent tool for researchers examining the therapeutic effects of cGAS. cGAS Screening Bundles. Bundle includes one Transcreener cGAMP cGAS TR-FRET Assay Kit (3025-10K, 10,000 Assay, 384 Well) and 200 μg Active, full-length Human Cyclic GMP-AMP synthase enzyme (cGAS enzyme), His-tagged protein expressed and purified from E.coli. For use with cGAS activity assays, screening, SAR, hit-to-lead, residence time determination, cGAS discovery, and more.|
|Restrictions:||Only available in Germany and selected European countries.|
|More information:||Go to webpage|
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