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High Throughput Autotaxin Inhibitor Screening Kit

High Throughput Autotaxin Inhibitor Screening Kit
Description: The High Throughput Autotaxin Inhibitor Screening Kit uses purified autotaxin (ATX) and the autotaxin substrate FS-3, which fluoresces upon cleavage. The assay is performed in a 384 well plate and quantifies the autotaxin activity in the presence of the user's samples by measuring changes in fluorescence over time. Product Background The High Throughput Autotaxin Inhibitor Screening Kit, developed by Echelon Biosciences Inc., uses the fluorogenic autotaxin substrate FS-3. FS-3 is an LPC analogue that is conjugated with both a fluorophore and a quencher. In its native state the quencher interferes with the fluorophore"s fluorescence. Once autotaxin cleaves FS-3, the fluorophore becomes liberated from the quencher, resulting in increased fluorescence. Percent autotaxin inhibition is determined by referencing no inhibitor controls. The autotaxin inhibitor, BrP-LPA, is supplied to positively control for autotaxin inhibition. The assay is resistant to modest concentrations of DMSO and is performed in a continuous assay format, which has the advantage of being less susceptible to error arising from mis-timing the addition of components to different sample wells.
Order #: K-4200HTS-EAKIT-EC
Unit Size: EA KIT
Supplier: Echelon Biosciences
Restrictions: Available in all European countries, except France & Italy.
Shipping: Dry Ice Or Gel Ice Depending On Destination
Storage: -20 °C
Subcategory: Kits & Assays
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