|Description:||The PI(5)P 4-Kinase (PIP4KII) Activity Assay is an ATP depletion assay which quantifies the remaining ATP levels in solution following the kinase reaction. The assay can be used to quantify PI(5)P 4-Kinase in vitro or in cell lysate. Sample Volume: 10 µL of the kinase samples per well Assay Incubation Time: 30 min to 2 hrs depending on enzyme reaction time Assay Range: 0.15 µM to 10 µM ATP Product Description Type II PI(5)P 4-Kinase (PIP4KII) catalyzes the phosphorylation of phosphatidylinositol-5-phosphate [PI(5)P] to phosphatidylinositol-4, 5-bisphosphate [PI(4, 5)P2]. PIP4KII has been shown to play a role in certain types of breast and colon cancer in addition to being important in several physiological processes such as insulin signaling, stress response, and embryonic development in several model organisms. It has also been implicated as an important regulator of nuclear PI(5)P levels. The assay reaction is prepared by the addition of PIP4KII (enzyme), PI(5)P (substrate) in reaction buffer and initiated by the addition of ATP. The reaction is then stopped by adding the ATP detector after the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. Echelon"s PI(5)P 4-Kinase (PIP4KII) Activity Assay is tested using SignalChem"s PIP4KIIA (P76-10AG) kinase.|
|Unit Size:||EA KIT|
|Restrictions:||Available in all European countries, except France & Italy.|
|Shipping:||Dry Ice Or Gel Ice Depending On Destination|
|Subcategory:||Kits & Assays|
|More information:||Go to webpage|
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 (0)551 707 22 70
- E-mail: firstname.lastname@example.org
How To Order
Orders can be placed by phone, fax, e-mail, or via our online shop:
After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.