PI(4)P 5-Kinase (PIP5KI) Activity Assay
| Description: | The PI(4)P 5-Kinase (PIP5K1) Activity Assay is an ATP depletion assay which quantifies the remaining ATP levels in solution following the kinase reaction. The assay can be used to quantify PIP5K1 activity in vitro or in cell lysate. Sample Volume: 10 µL of the kinase samples per well Assay Incubation Time: 30 min to 2 hrs depending on enzyme reaction time Assay Range: 0.15 µM to 10 µM ATP Product Background Type I PI(4)P 5-Kinase (PIP5KI) catalyzes the phosphorylation of phosphatidylinositol-4-phosphate [PI(4)P] to phosphatidylinositol-4, 5-bisphosphate [PI(4, 5)P2]. PIP5KI has been shown to play a role in several cellular functions including gene transcription, actin cytoskeleton reorganization, secreation and endocytosis. It is thought that PIP5KI activity is responsible for the majority of the PI(4, 5)P2 found in the cell which is an important second messenger for many cellular processes. The assay reaction is prepared by the addition of PIP5KI (enzyme), PI(4)P (substrate) in reaction buffer and initiated by the addition of ATP. The reaction is then stopped by adding the ATP detector after the chosen reaction time. The luminescent signal is inversely correlated with the kinase activity. Echelon"s PI(4)P 5-Kinase Activity Assay is tested using SignalChem"s PIP5KIA (P16-10AG) kinase. |
| Order #: | K-5700-EAKIT-EC |
| Unit Size: | EA KIT |
| Supplier: | Echelon Biosciences |
| Restrictions: | Available in all European countries, except France & Italy. |
| Shipping: | Dry Ice Or Gel Ice Depending On Destination |
| Storage: | -20 °C |
| Subcategory: | Kits & Assays |
| More information: | Go to webpage |
728.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 551 707 220
- Fax: +49 551 707 2222
- E-mail: info@mobitec.com
- Online Shop: www.mobitec.com
- Phone: +49 (0)551 707 22 70
- E-mail: info@mobitec.com
How To Order
Orders can be placed by phone, fax, e-mail, or via our online shop:
After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.
Technical Service - Product Information
Viewed