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LPLA2 Inhibitor Screen

LPLA2 Inhibitor Screen
Description: Human lysosomal phospholipase A2 (LPLA2) is responsible for normal lipid metabolism and it is unique from other known PLA2s in that LPLA2 is only active in an acidic environment (~pH 4.5). Phospholipidosis (PL) is a condition resulting from the excessive accumulation of intracellular phospholipids, causing tissue inflammation and organ damage. PL can manifest in patients taking certain cationic amphiphilic drugs (CADs) such as fluoxetine (Prozac™, Sarafem) and Amiodarone. The FDA has determined that drug-induced phospholipidosis (DIPL) is a serious drug safety issue and evidence is accumulating that DIPL is the result of certain CADs directly inhibiting LPLA2. Echelon’s LPLA2 Inhibitor Screen (K-7000I) is designed to assay a drug’s ability to inhibit LPLA2 activity in vitro, and thus potentially predict DIPL. The assay uses a quenched substrate which fluoresces when hydrolyzed by LPLA2. This direct biochemical approach provides a quantitative measurement in a robust and simple-to-use in vitro plate-based assay, providing greatly increased throughput when compared to traditional microscopy methods of tissue cultured cells. The assay has been validated with a group of known PL-inducing and non-PL inducing drugs. The known PL-inducing cationic amphiphilic drug, amiodarone, is included in the assay as a control.
Order #: K-7000I-EC
Unit Size: EA KIT
Supplier: Echelon Biosciences
Restrictions: Available in all European countries, except France & Italy.
Shipping: See Manual
Storage: See Manual
Subcategory: Kits & Assays
More information: Go to webpage
Datasheet
811.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 days

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