SensoLyte® 520 Cathepsin L Assay Kit Fluorimetric - 1 kit
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| Description: | Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins. The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL™ 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity. |
| Order #: | AS-72218- |
| Unit Size: | 1 kit |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | Dry Ice |
| Storage: | - 78 °C |
| Subcategory: | Enzyme Assay Kits |
| More information: | Go to webpage |
582.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
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