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SensoLyte® 520 Granzyme B Activity Assay Kit, Fluorimetric

Description: Granzyme B is a serine protease mostly secreted by cytotoxic T lymphocyte (CTL) and natural killer (NK) cells. It is one of the major mediators of caspase-dependent apoptosis. Recent studies showed that Granzyme B can also be secreted by various non-cytotoxic cells and involved in degradation of extracellular matrix proteins inducing inflammation and impairing wound healing. Thus if intracellular activity of Granzyme B is mainly related to apoptosis its extracellular activity has been linked to arthritis, vascular pathologies, and other diseases. The SensoLyte® 520 Granzyme B Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of Granzyme B activity using a novel internally quenched 5-FAM/QXLTM FRET substrate. Upon cleavage by Granzyme B, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The kit can be used to detect the activity of Granzyme B enzyme in biological samples and purified enzyme preparations. This assay can detect as low as 0.1 ng/mL active granzyme B. The kit contains: • QXL® 520/ 5-FAM-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) • Assay buffer • Recombinant Human Granzyme B • Inhibitor • Fluorescence reference standard for calibration • A detailed protocol Kit size: 100 assays (96-well plate)
Order #: AS-72261
Unit Size: 1 kit
Supplier: AnaSpec
shipping: Blue ice
storage: -20°C
restrictions: Only available in Germany and selected European countries.
Product Subcategory: Enzyme Assays
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501.00 € *

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Delivery time approx. 8 – 10 days

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