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SensoLyte® 490 HIV Protease Assay Kit *Fluorimetric*

Description: HIV protease (PR) is identified as an important drug-screening target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The SensoLyte® 490 HIV Protease Assay Kit uses an optimized FRET peptide substrate for a continuous measurement of HIV protease activities. This FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR with this substrate results in specific cleavage and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this kit offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. The kit contains:EDANS/DABCYL-based FRET peptide substrate (Ex/Em=340/490 nm upon cleavage), Assay buffer, HIV protease inhibitor, Fluorescence reference standard for calibration, A detailed protocol
Order #: AS-71127
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Blue Ice
Storage: -20°C
Subcategory: Enzyme Assays
More information: Go to webpage
Datasheet
631.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 days

    How To Order

    Orders can be placed by phone, fax, e-mail, or via our online shop:

    After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.

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