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SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*

SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*
Description: The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement ofEnzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: 5-FAM/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Fluorescence reference standard for calibration, A detailed protocol
Order #: AS-71145
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Blue Ice
Storage: -20°C
Subcategory: Enzyme Assays
More information: Go to webpage
457.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

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