SensoLyte® 520 MMP-12 Assay Kit Fluorimetric - 1 kit
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| Description: | Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is expressed in activated macrophages and may play a role in smoke-induced emphysema. SensoLyte® 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. |
| Order #: | AS-71157 |
| Unit Size: | 1 kit |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | 4 °C |
| Storage: | - 20 °C |
| Subcategory: | Enzyme Assay Kits |
| More information: | Go to webpage |
554.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
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