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SensoLyte® 520 Renin Assay Kit *Fluorimetric*

SensoLyte® 520 Renin Assay Kit *Fluorimetric*
Description: Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I, which is further converted into angiotensin II by ACE (Angiotensin ConvertingEnzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. It also increases the secretion of ADH and aldosterone, and stimulates the hypothalamus to activate the thirst reflex. Since an overactive renin-angiotensin system leads to hypertension, renin is an attractive target for the treatment of this disease. The SensoLyte® 520 Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a 5-FAM/QXL®520 fluorescence resonance energy transfer (FRET) peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL®520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay and can detect 0.8 ng/ml renin. The kit contains:, 5-FAM/QXL®520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Assay buffer, Human recombinant renin, Renin inhibitor, Fluorescence reference standard for calibration, A detailed protocol
Order #: AS-72040
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Dry Ice
Storage: Component C at -80 °C, all other components at -20 °C
Subcategory: Enzyme Assays
More information: Go to webpage
Datasheet
599.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 days

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