SensoLyte® 390 Cathepsin D Assay Kit *Fluorimetric*
| Description: | Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. It is involved in the pathogenesis of several diseases such as breastCancer and Alzheimer disease. The SensoLyte® 390 Cathepsin D Activity Assay Kit uses a Mca/Dnp FRET peptide for measurement ofEnzyme activity. In the FRET peptide, the fluorescence of Mca is quenched by Dnp. Upon cleavage into two separate fragments by Cathepsin D, the fluorescence of Mca is recovered, and can be monitored at excitation/emission = 330 nm/390 nm. The kit contains: , Mca/Dnp-based FRET peptide substrate (Ex/Em=330 nm/390 nm upon cleavage) , Assay buffer , Bovine spleen Cathepsin D , Inhibitor , Fluorescence reference standard for calibration , A detailed protocol |
| Order #: | AS-72098 |
| Unit Size: | 1 kit |
| Supplier: | AnaSpec |
| Restrictions: | Only available in Germany and selected European countries. |
| Shipping: | Dry Ice |
| Storage: | Component C at -80 °C, all other components at -20 °C |
| Subcategory: | Enzyme Assays |
| More information: | Go to webpage |
413.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 days
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