|Description:||β-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporterEnzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® ONPG β-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl β-D-galactopyranoside (ONPG) to determine the activity of β-galactosidase in transfected cells or tissues. In the presence of β-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to β-galactosidase activity. The kit contains: b-galactosidase substrate, β-galactosidase standard, reaction buffer, stop buffer and optimized assay protocol. Assays are performed in a convenient 96-well format. Kit size: 500 assays|
|Unit Size:||1 kit|
|Restrictions:||Only available in Germany and selected European countries.|
|More information:||Go to webpage|
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Delivery time approx. 8 - 10 working days
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