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SensoLyte® ONPG β-Galactosidase Assay Kit *Colorimetric*

SensoLyte® ONPG β-Galactosidase Assay Kit *Colorimetric*
Description: β-galactosidase, encoded by the lacZ gene in E. coli, is widely used as a reporterEnzyme to study gene expression, protein-protein interaction and normalization of transfection efficiency in mammalian cells. The SensoLyte® ONPG β-Galactosidase Assay Kit utilizes the widely used chromogenic substrate, o-nitrophenyl β-D-galactopyranoside (ONPG) to determine the activity of β-galactosidase in transfected cells or tissues. In the presence of β-galactosidase, the colorless compound is hydrolyzed into o-nitrophenol, producing a yellow color that can be detected at absorbance 420 nm. The intensity of the color produced is proportional to β-galactosidase activity. The kit contains: b-galactosidase substrate, β-galactosidase standard, reaction buffer, stop buffer and optimized assay protocol. Assays are performed in a convenient 96-well format. Kit size: 500 assays
Order #: AS-72134
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Blue Ice
Storage: -20°C
Subcategory: Enzyme Assays
More information: Go to webpage
Datasheet
223.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

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