|Description:||Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown. Cathepsin D is involved in the pathogenesis of several diseases such as breastCancer and Alzheimer disease. The SensoLyte® 520 Cathepsin D Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measurement ofEnzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the activeEnzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin DEnzyme in biological samples and purifiedEnzyme preparations. The kit contains:, HiLyte™ Fluor 488/QXL®520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Assay buffer, Bovine spleen Cathepsin D, Inhibitor, Fluorescence reference standard for calibration, A detailed protocol Kit size: 100 assays (96-well plate)|
|Unit Size:||1 kit|
|Restrictions:||Only available in Germany and selected European countries.|
|Storage:||Component C at -80 °C, all other components at -20 °C|
|More information:||Go to webpage|
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