|Description:||Cathepsins are a class of globular lysosomal proteases playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin K is the lysosomal cysteine protease involved in bone remodeling and resorption. It has potential as a drug target in autoimmune diseases and osteoporosis. The SensoLyte® 520 Cathepsin K Activity Assay Kit uses a QXL® 520/HiLyte™ Fluor 488 FRET peptide substrate for the measurement ofEnzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by the activeEnzyme, the increase of fluorescence can be continuously monitored at excitation/emission = 490/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, which is optimal for cathepsin activity. The kit can be used to detect the activity of Cathepsin KEnzyme in biological samples and purifiedEnzyme preparations. The kit contains:, QXL® 520/HiLyte™ Fluor 488-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Assay buffer, Procathepsin K, Activation buffer, Inhibitor, Fluorescence reference standard for calibration, A detailed protocol Kit size: 100 assays (96-well plate)|
|Unit Size:||1 kit|
|Restrictions:||Only available in Germany and selected European countries.|
|Storage:||Component C at -80 °C, all other components at -20 °C|
|More information:||Go to webpage|
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