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SensoLyte® 520 Enterokinase Activity Assay Kit *Fluorimetric*

SensoLyte® 520 Enterokinase Activity Assay Kit *Fluorimetric*
Description: Enterokinase is a heterodimeric serine protease produced by cells in the duodenal wall. It converts trypsinogen to trypsin, indirectly activating a number of pancreatic digestiveEnzymes. Enterokinase is a keyEnzyme for intestinal digestion of proteins. Its deficiency may cause severe protein malabsorption. Enterokinase also plays an important role in acute experimental and clinical pancreatitis. The high degree of specificity exhibited by enterokinase for cleaving affinity or other tags from recombinant proteins makes it an ideal tool for use in biochemical applications. The SensoLyte® 520 Enterokinase Assay Kit is optimized for detecting activity of enterokinase. This kit employs a novel internally quenched 5-FAM/QXL FRET substrate for enterokinase. Enterokinase cleaves the FRET substrate into two separate fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 1.25 ng/mL of active enterokinase. Kit size: 100 assays
Order #: AS-72209
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Dry Ice
Storage: Component C at -80 °C, all other components at -20 °C
Subcategory: Enzyme Assays
More information: Go to webpage
Datasheet
501.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

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