|Description:||Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins. The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL™ 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.|
|Unit Size:||1 kit|
|Restrictions:||Only available in Germany and selected European countries.|
|Storage:||Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.|
|Subcategory:||Enzyme Assay Kits|
|More information:||Go to webpage|
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