|Description:||Sortases are a family of membrane-anchored transpeptidases expressed by Gram-positive bacteria. Sortase A catalyzes the cleavage of C-terminal recognition motif (LPXTG) of multiple structurally unrelated proteins followed by formation of an amide bond with the peptidoglycan layer of the bacteria. Since sortases are widely distributed among a variety of bacterial pathogens and required for virulence, they represent a promising therapeutic target for the development of novel anti-infective agents. In addition, Sortase A can be used as a biotechnology tool for a variety of protein modifications and immobilization by sortase-mediated protein ligation. The SensoLyte® 520 Sortase A Assay Kit employs a novel internally quenched 5-FAM/QXL® FRET substrate for the detection of protease activity. The enzyme cleaves the FRET substrate into two separate fragments resulting in the release of 5-FAM fluorescence, which can be monitored at excitation/emission= 490/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds.|
|Unit Size:||1 kit|
|Restrictions:||Only available in Germany and selected European countries.|
|Storage:||Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.|
|Subcategory:||Enzyme Assay Kits|
|More information:||Go to webpage|
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