mobitec-globe Innovative Tools for Molecular and Cell Biology

SensoLyte® 520 ECEs Activity Assay Kit Fluorimetric - 1 kit

SensoLyte® 520 ECEs Activity Assay Kit Fluorimetric - 1 kit
Usage: hover your mouse-pointer anywhere on the image above to see an enlarged portion of the image. You can also just click the image to see the original-sized version.
Description: Endothelin converting enzymes (ECEs), similar to neprilysin (NEP), belong to the M13 family of zinc metallopeptidases. ECEs (ECE-1/ECE-2) are known as the rate-limiting enzymes in the formation of the vasoconstrictor peptide endothelin-1 (ET-1) from its precursor proendothelin-1. ECE-1 can also degrade amyloid fragments both in vitro and in vivo. Highly homologous and structurally comparable to ECE-1, ECE-2 was shown in homozygous knockout mice to have decreased Abeta degradation and significant elevated Abeta 1-40 and 1-42 in the brain. Some studies indicate that carriers of ECE1 and ECE-2 gene variants may have an increased risk of developing late-onset Alzheimer’s disease (AD). However, the roles of ECE-1 and ECE-2 in AD progression have yet to be fully elucidated. The SensoLyte® 520 ECEs Activity Assay Kit can be used to detect enzyme activity in biological samples or in purified enzyme preparations. When active ECEs cleave the FRET substrate, it results in an increase of 5-FAM fluorescence, and is monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM shows less interference from autofluorescence of components in biological samples and test compounds. This assay can detect as low as 15.6 ng/mL active ECE-1 and 62.5 ng/mL active ECE-2.
Order #: AS-72243
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Dry Ice
Storage: - 78 °C
Subcategory: Enzyme Assay Kits
More information: Go to webpage
Datasheet Flyer or Brochure
607.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

    How To Order

    Orders can be placed by phone, fax, e-mail, or via our online shop:

    After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.

    Technical Service - Product Information