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SensoLyte® Transglutaminase Activity Assay Kit, Colorimetric

SensoLyte® Transglutaminase Activity Assay Kit, Colorimetric
Description: Transglutaminases (TGs) are Ca2+ and thiol-dependentEnzymes that catalyze posttranslational modifications of proteins. These covalent reactions include formation of isopeptide linkages between the carboxamide groups of protein-bound glutamine residues and ε-amino groups of protein-bound lysine and polyamine residues, hydrolysis and esterification of glutamine residues. Family of TGs includes at least nine members: TG 1-7, coagulation factor XIIIa, and the keratinocyte membrane bound TG form. TG has attracted considerable interest as a potential drug target. Its activity is thought to contribute to Alzheimer disease, Parkinson disease, Huntington disease, and supranuclear palsy. SensoLyte® Transglutaminase Activity Assay Kit provides a convenient assay for the high throughput screening of TG modulators and inhibitors. TG catalyzes covalent bond formation between a free amine group of poly-D-lysine, which is coated on the plate surface, and γ-carboxamide group of glutamine in biotin-TVQQEL peptide substrate. The reaction immobilizes biotin-conjugated peptide to the plate surface. The amount of immobilized biotin is determined using streptavidin-horseradish peroxidase (HRP) andTMB substrate. Colorimetric signal generated by HRP and TMB substrate is proportional to the activity of TG in a sample. Assay is performed in a convenient 96-well microplate format.
Order #: AS-72244
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Dry Ice
Storage: Component D at -80 °C, all other components at -20 °C
Subcategory: Enzyme Assays
More information: Go to webpage
Datasheet
457.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

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