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SensoLyte® 520 Meprin Alpha Activity Assay Kit *Fluorimetric*

SensoLyte® 520 Meprin Alpha Activity Assay Kit *Fluorimetric*
Description: Meprin Alpha can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin, SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin Alpha is associated withCancer proliferation,Cancer migration and invasion. The FRET substrate included in SensoLyte® 520 Meprin Alpha Activity Assay Kit was designed to detect Meprin Alpha activity and reduce the cross reactivity with Meprin Beta, ADAM10, Alpha -secretase, BACE-2, ECEs and TACE. This assay kit can be used to detectEnzyme activity in purifiedEnzyme preparations and compound screening. Active Meprin Alpha cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin Alpha. The kit contains: • 5-FAM/QXL™ 520 Meprin Alpha substrate, Ex/Em=490/520 nm upon cleavage • Assay buffer, Recombinant Human Meprin Alpha • Meprin Alpha activation buffer • Meprin Alpha stop solution • Inhibitor • Fluorescence reference standard for calibration, A detailed protocol Kit size: 100 assays (96-well plate)
Order #: AS-72253
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Dry Ice
Storage: Component C at -80 °C, all other components at -20 °C
Subcategory: Enzyme Assays
More information: Go to webpage
501.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 working days

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