mobitec-globe Innovative Tools for Molecular and Cell Biology

SensoLyte® 520 Meprin Alpha Activity Assay Kit *Fluorimetric*

SensoLyte® 520 Meprin Alpha Activity Assay Kit *Fluorimetric*
Usage: Hover your mouse-pointer anywhere on the image above to see an enlarged portion of the image. You can also just click the image to see the original-sized version.
Description: Meprin Alpha can cleave extracellular matrix proteins (pro-collagen I, fibronectin, osteopontin, SPARC), growth factors (VEGF-A, CTGF) and proteinases (MMP-1). Evidence has suggested that Meprin Alpha is associated withCancer proliferation,Cancer migration and invasion. The FRET substrate included in SensoLyte® 520 Meprin Alpha Activity Assay Kit was designed to detect Meprin Alpha activity and reduce the cross reactivity with Meprin Beta, ADAM10, Alpha -secretase, BACE-2, ECEs and TACE. This assay kit can be used to detectEnzyme activity in purifiedEnzyme preparations and compound screening. Active Meprin Alpha cleaves the FRET substrate, resulting in an increase of 5-FAM fluorescence, monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.1 ng/mL active Meprin Alpha. The kit contains: • 5-FAM/QXL™ 520 Meprin Alpha substrate, Ex/Em=490/520 nm upon cleavage • Assay buffer, Recombinant Human Meprin Alpha • Meprin Alpha activation buffer • Meprin Alpha stop solution • Inhibitor • Fluorescence reference standard for calibration, A detailed protocol Kit size: 100 assays (96-well plate)
Order #: AS-72253
Unit Size: 1 kit
Supplier: AnaSpec
Restrictions: Only available in Germany and selected European countries.
Shipping: Dry Ice
Storage: Component C at -80 °C, all other components at -20 °C
Subcategory: Enzyme Assays
More information: Go to webpage
Datasheet
501.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 days

    How To Order

    Orders can be placed by phone, fax, e-mail, or via our online shop:

    After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.

    Technical Service - Product Information
Viewed