|Description:||Meprin Beta, a type I transmembrane metalloprotease, belongs to the astacin family of zinc endopeptidases and the metzincin superfamily. Protein expression has been identified in multiple tissues and cell types such as kidney, intestine, skin, leukocytes and severalCancer cells. Though the biological function of Meprin Beta is not fully understood, it was found that Meprin Beta plays various roles in inflammatory response, collagen assembly,Cancer progression and beta-amyloid protein cleavage. More growing evidence has indicated that Meprin Beta is a good therapeutic target in regulating angiogenesis,Cancer, inflammation, fibrosis, and neurodegenerative diseases. The SensoLyte® 520 Meprin Beta Activity Assay Kit can be used to detectEnzyme activity in purifiedEnzyme preparations and compound screening. The unique FRET substrate was derived from APP sequence designed to reduce the cross reactivity with Meprin alpha, ADAM10, Alpha-secretase, BACE-2, IDE and ECEs, Neprilysin and TACE. Active Meprin Beta cleaves the FRET substrate resulting in an increase of 5-FAM fluorescence, that can be monitored at excitation/emission = 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is also less interfered by the autofluorescence of components in biological samples and test compounds. This assay can detect as low as 0.048 ng/mL active Meprin Beta. The kit contains:• 5-FAM/QXL™ 520 Meprin Beta substrate, Ex/Em=490/520 nm upon cleavage • Assay buffer, Recombinant Human Meprin Beta • Meprin Beta activation buffer • Meprin Beta stop solution • Inhibitor • Fluorescence reference standard for calibration, A detailed protocol Kit size: 100 assays (96-well plate)|
|Unit Size:||1 kit|
|Restrictions:||Only available in Germany and selected European countries.|
|Storage:||Component C at -80 °C, all other components at -20 °C|
|More information:||Go to webpage|
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 days
- Phone: +49 (0)551 707 22 70
- E-mail: firstname.lastname@example.org
How To Order
Orders can be placed by phone, fax, e-mail, or via our online shop:
After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.