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T4 β-Glucosyltransferase

T4 β-Glucosyltransferase
Description: T4 β-glucosyltransferase (T4 BGT) is a DNA-modifying enzyme encoded by bacteriophage T4 that catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, resulting in the formation of β-glucosyl-5- hydroxymethylcytosine. The enzyme is available in 500- and 2,500-unit sizes at a concentration of 10 U/µL. The enzyme is supplied with a 10× Reaction Buffer. Applications: • Glucosylation or immunodetection of 5-hmC DNA. • Differentiation of 5-hmC from 5-mC. 5-hmC containing DNA enrichment. • Labeling of 5-hmC residues using a radioactive UDP-glucose donor.
Order #: E100-2-ABP
Unit Size: 2500 Units
Supplier: ABP Biosciences
Shipping: Ice Pack
Storage: -20°C
Subcategory: Enzymes
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