|Description:||T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The T4 DNA Ligase requires ATP as a cofactor. The enzyme is available in 250 and 1,250 unit sizes at a concentration of 5 U/µL. The enzyme is supplied with a 10× T4 Ligation Buffer. Applications: • Routine subcloning. • Recircularization of linear DNA. • Library construction. • Linker ligation.|
|Unit Size:||1250 Units|
|More information:||Go to webpage|
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