|Description:||Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reaction makes tailing more efficient. The enzyme is available in 500- and 2,500-unit sizes at a concentration of 20 U/µL. The enzyme is supplied with a 10× Reaction Buffer and 2.5 mM CoCl2. Applications: • Addition of homopolymer tails to the 3´ ends of DNA. • Labeling the 3´ ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP) • TUNEL asay (in situ localization of apoptosis) • TdT dependent PCR.|
|Unit Size:||500 Units|
|More information:||Go to webpage|
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