|Description:||The kit is designed for rapid fractionation of fresh/frozen soft plant tissues, especially leaves. Unlike other commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy processing time are required, this kit only uses 100-200 mg starting material. By using a specialized filter cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol, nuclei, chloroplasts and organelles) can be obtained in about 1 hour. The subcellular fractions can be used in numerous applications that include but not limited to protein trafficking, nuclear protein extraction and nucleic acid purification/sequencing. The highly enriched nuclei can be used for proteomics, flow cytometry analysis, DNA sequencing, and protein-protein/protein-DNA interaction studies. The subcellular fractionation protocol includes grinding tissue to release plant cells followed by disruption of the cell membrane by rapidly passing through the filter. Different subcellular fraction is obtained by differential centrifugation using only a table top centrifuge. This kit has been tested for use with many species such as Arabidopsis, Spinach, Peas, and Rape.|
|Unit Size:||20 Tests|
|Restrictions:||Only available in Germany and selected European countries.|
|More information:||Go to webpage|
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 days
- Phone: +49 (0)551 707 22 70
- E-mail: email@example.com
How To Order
Orders can be placed by phone, fax, e-mail, or via our online shop:
After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.