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Minute™ Rubisco Depletion Kit

Minute™ Rubisco Depletion Kit
Description: Plants perceive signals from the environment through leaves. One of the major goals of the scientific community is to dissect the components of signaling cascades by analyzing the leaf proteome. However, the major bottleneck is presence of a high-abundant protein —Rubisco, which accounts for majority of leaf protein ranging from 40-80%. High level rubisco masks the detection and identification of low abundant proteins by LC-MS/MS-based method. A specific IgY conjugated resin is available commercially for depletion of rubisco from extracted total protein but only small amount of protein can be handled and it is very costly. Other methods have been described in the literatures but are usually tedious and time consuming. The MinuteTM Rubisco Depletion Kit is designed to overcome these disadvantages by using a specialized filter cartridge coupled with proprietary rubisco depletion buffer. The same, if not better, results can be obtained with a much easier and straighter forward protocol in about 1h. The rubisco-depleted total protein can be used for many downstream applications such as SDS-PAGE, 2DE and LC MS/MS analysis.Plants perceive signals from the environment through leaves. One of the major goals of the scientific community is to dissect the components of signaling cascades by analyzing the leaf proteome. However, the major bottleneck is presence of a high-abundant protein —Rubisco, which accounts for majority of leaf protein ranging from 40-80%. High level rubisco masks the detection and identification of low abundant proteins by LC-MS/MS-based method. A specific IgY conjugated resin is available commercially for depletion of rubisco from extracted total protein but only small amount of protein can be handled and it is very costly. Other methods have been described in the literatures but are usually tedious and time consuming. The MinuteTM Rubisco Depletion Kit is designed to overcome these disadvantages by using a specialized filter cartridge coupled with proprietary rubisco depletion buffer. The same, if not better, results can be obtained with a much easier and straighter forward protocol in about 1h. The rubisco-depleted total protein can be used for many downstream applications such as SDS-PAGE, 2DE and LC MS/MS analysis.Plants perceive signals from the environment through leaves. One of the major goals of the scientific community is to dissect the components of signaling cascades by analyzing the leaf proteome. However, the major bottleneck is presence of a high-abundant protein —Rubisco, which accounts for majority of leaf protein ranging from 40-80%. High level rubisco masks the detection and identification of low abundant proteins by LC-MS/MS-based method. A specific IgY conjugated resin is available commercially for depletion of rubisco from extracted total protein but only small amount of protein can be handled and it is very costly. Other methods have been described in the literatures but are usually tedious and time consuming. The MinuteTM Rubisco Depletion Kit is designed to overcome these disadvantages by using a specialized filter cartridge coupled with proprietary rubisco depletion buffer. The same, if not better, results can be obtained with a much easier and straighter forward protocol in about 1h. The rubisco-depleted total protein can be used for many downstream applications such as SDS-PAGE, 2DE and LC MS/MS analysis.
Order #: RD-046
Unit Size: 20 Tests
Supplier: Invent Biotechnologies
Restrictions: Only available in Germany and selected European countries.
Shipping: Ambient
Storage: 4 °C
Subcategory: Protein Extraction
More information: Go to webpage
Datasheet
318.00 € *

*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).

Delivery time approx. 8 - 10 days

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    After placing your order, you will receive an order confirmation via e-mail within 2 working days. If you do not receive this e-mail, please contact us by phone. Thank you! More information regarding our ordering process can be found here.

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