MAGNO-sorb-URO (Ecoli Dx Product Code: K4-2181-100-CE)
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| Description: | Nucleic Acid Extraction Kits – AmpliSens MAGNO-sorb-URO nucleic acid extraction kit is intended for extraction of DNA from biological material (vaginal, cervical, urethral discharge; urine) for subsequent qualitative or quantitative detection of DNA of pathogens which causes sexually transmitted infections, other infections of reproductive organs and urinary tract by the polymerase chain reaction (PCR). The reagent kit can be used in combination with the automatic station for the nucleic acids extraction providing that the procedure described in this instruction manual is programmed. DNA extraction is preanalytical phase of PCR method in the clinical laboratory diagnostics. The volume of test sample for the extraction is 100 μl. A test sample1 is treated by the lysis solution in the presence of the magnetic silica particles (magnetic sorbent). As a result, the cell membranes, viral envelopes and other biopolymer complexes are destructed and DNA is released. The dissolved DNA binds to the sorbent particles while other components of the lysed biological material stay in the solution and are removed by sorbent sedimentation at a magnetic rack and subsequent washings. The DNA is transferred from the sorbent surface to the solution after adding the buffer for elution to the magnetic sorbent. Then the solution is separated from the sorbent by magnetic force. The obtained DNA sample is highly purified and free from inhibitors of amplification, that provides high analytical sensitivity of PCR assay. |
| Order #: | ECO-K4-2181-100-CE |
| Unit Size: | 100 |
| Supplier: | Ecoli Dx |
| Restrictions: | Only available in selected countries. |
| Shipping: | See Manual |
| Storage: | See Manual |
| Subcategory: | PCR and Real-Time PCR Diagnostics |
| More information: | Go to webpage |
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