DNA-sorb-D (Ecoli Dx Product Code: K8-2331-100-CE)
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| Description: | Nucleic Acid Extraction Kits – AmpliSens DNA-sorb-D nucleic acid extraction kit is intended for extraction of DNA from epithelial cells (cervical swabs) taken into the transport medium for liquid-based cytology (for example, PreservCyt (Hologic Inc., USA)) for subsequent analysis by the polymerase chain reaction (PCR). Test samples are treated using mucolysin to dissolve cervical mucus. Then the cells are washed out of the transport medium using phosphate buffer solution (PBS-buffer) and cells membranes are destructed by lysis with detergent (cytolysin). As a result, the nucleic acids are released and the proteins are degraded by protease. DNA without proteins is purified by adsorption on silica gel. The dissolved nucleic acids bind to the sorbent particles while other components of the lysed biological material stay in the solution and are removed by sorbent centrifugal sedimentation and subsequent washings. The nucleic acids are transferred from the silica surface to the solution after adding the buffer for elution to the sorbent. Then the solution is separated from the sorbent by centrifugation. The obtained nucleic acid sample is highly purified and free from inhibitors of amplification, which provides high analytical sensitivity of PCR assay. |
| Order #: | ECO-K8-2331-100-CE |
| Unit Size: | 100 |
| Supplier: | Ecoli Dx |
| Restrictions: | Only available in selected countries. |
| Shipping: | See Manual |
| Storage: | See Manual |
| Subcategory: | PCR and Real-Time PCR Diagnostics |
| More information: | Go to webpage |
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