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3CLglow - Live Cell Fluorescence Assay for 3CL Protease

Evaluate Therapeutic Drugs that Target SARS-CoV-2 Replication

The 3CLglow Assay is a SARS-CoV-2 3CLpro inhibitor assay, a new technology developed by Montana Molecular in response to the COVID-19 pandemic. This assay provides a robust, biologically meaningful tool to evaluate therapeutic drugs that target SARS-CoV-2 replication.

  • 3C-like protease (3CLpro, Mpro, or Main protease) is essential for SARS-CoV-2 replication
  • Discover new 3CL protease inhibitors
  • A single BacMam delivers a gene encoding the green fluorescent 3CLglow Sensor as well as constitutively expressed red protein to check cell health
  • BSL-1 kit is safe and easy to use

Simple Assay Steps

  • Day 1 - Plate 35,000 HEK293 cells in each well
  • Day 2 - Make transduction mix by combining 3CLglow BacMam, 3CLpro Enzyme, complete media, and sodium butyrate (included in 3CLglow Assay Kit)
  • Day 3 - Add inhibitor compounds and measure green and red fluorescence in either an endpoint assay to maximize throughput, or a kinetic assay for real-time detection of inhibitor action in living cells

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Download Protocol

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Related Products:

COVID-19 Drug Screening / Pseudovirus Neutralization Assay

  • Fluorescent SARS-CoV-2 cell entry assay based on two components: The first is a BacMam expression vector coding for human ACE2 to transform any cell into pseudo host cells. The second assay component is a SARS-CoV-2 Pseudovirus - a modified BacMam presenting the Spike protein on the surface of the BacMam capsid
  • Identify therapeutic drugs that block Spike ACE2 binding
  • No BSL-3 safety requirements
  • Screen for neutralizing antibodies to SARS-CoV-2 spike and emerging spike variants
  • BacMam is high-titer and more cost-effective to use than Lentivirus-based assays

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