Fluorescent Assays for SARS-CoV-2 Viral Entry
- Detect host-pathogen interaction in living cells
- Identify therapeutic drugs that block ACE2-mediated viral entry
- Screen for neutralizing antibodies to SARS-CoV-2 spike and emerging spike variants
- Cost effective screening with minimal safety requirements
- Your choice of host cell
This pseudovirus is a modified BacMam vector that presents SARS-CoV-2 spike protein on the surface of the BacMam capsid. The spike protein facilitates entry into cells expressing ACE2 and TMPRSS2. Once inside the cell, BacMam does not replicate, but delivers a genetically encoded, fluorescent reporter that expresses bright red or green fluorescence in the host cell nucleus. When viral entry is successfully blocked, the host cell nucleus is dark. Pseudo SARS-CoV-2 is safe to use with no risk of infection and requires only BSL-1 handling.
Pseudo Host Cells
To transform any cell into pseudo host cells, we offer red and green fluorescent ACE2 and TMPRSS2 in BacMam expression vectors. After transduction host cells are ready for the viral entry assay. If the pseudovirus enters the cells, it will produce bright fluorescence in the nucleus. A significant advantage of creating pseudo host cells is that compounds blocking viral entry can be screened using standard cell lines that are compatible with high-throughput screening.
COVID-19 Reagents – Safely screen SARS-CoV-2 blockers and protease inhibitors.
SARS-CoV-2 Entry – Host Cell Factors for SARS-CoV-2 Viral Entry
SARS CoV-2 Pseudoviruses – Tools for Measuring SARS-CoV-2 Entry
For the latest updates about COVID-19 tools for research and drug discovery, visit the NEWS page of Montana Molecular
Product Details: Pseudo SARS-CoV-2 Red Reporter
Product Details: Pseudo SARS-CoV-2 Green Reporter
Product Details: Pseudo SARS-CoV-2 D614G Mutation Red Reporter
Product Details: Pseudo SARS-CoV-2 D614G Mutation Green Reporter
Product Details: Angiotensin Converting Enzyme 2 (ACE2)-Green
Product Details: Angiotensin Converting Enzyme 2 (ACE2)-Red
Product Details: TMPRSS2
Product Details: Neuropilin 1
Product Details: Cathepsin L