NanoMolar Nickel / Cobalt Assay Kit (ProFoldin Product Code: NMA1000)
| Description: | Metal Ions ׀ Nickel and Cobalt Assays ׀ Nickel (Ni++) and Cobalt (Co++) are essential metal ions in biological systems. Many enzymes such as methionine aminopeptidase and glucose isomerase contain cobalt. Some other enzymes such as ureases from bacteria and plants use nickel as a cofactor. Synthesis of Ni / Co enzymes and coenzyme B12 requires high-affinity uptake of the metal ions from natural environments. In bacteria, Ni and Co uptake is mediated by secondary transporters and ATP-binding cassette systems. Understanding the differences between cobalt and nickel transporters might lead to drug development for gastritis and peptic ulceration. The NanoMolar Nickel / Cobalt Assay Kit is for measurement of nanomolar concentrations of nickel or cobalt (10 nM – 500 nM). The assay is based on the principle that binding the fluorescence dye NMA with nickel or cobalt ions results in decrease of the fluorescence intensity (emission 535 nm, excitation 485 nm). Other metal ions including Mg2+, Ca2+, Zn2+, Mn2+, Al3+, Cu2+ and Ag+ give much lower assay sensitivity. Chelators such EDTA and thiol compounds bind strongly some of the metal ions and should be avoided in the assay. The assay kit can be used for high-throughput measurements of nickel or cobalt concentrations in biological samples or environmental water samples. The kit can also be used for biochemical assays of enzyme assays associated with nickel or cobalt metabolism. This product includes 300 ul of 100 x NMA dye. It is for 1000 assays using 384-well plates (30 ul of sample volume) or 500 assays using 96-well plates (60 ul of sample volume). Cuvettes may also be used for measurements. |
| Order #: | PFD-NMA1000 |
| Unit Size: | 1000 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Concentration Measurement |
| More information: | Go to webpage |
505.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 (0)89 3799666-6
- Fax: +49 (0)89 3799666-99
- E-mail: order@biozol.de
- Online Shop: www.mobitec.com
- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
How To Order
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You will receive an order confirmation by fax or e-mail. More information regarding the ordering process can be found here.
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