NanoMolar Phosphate Assay Kit (ProFoldin Product Code: NPA1000)
| Description: | Salt and Buffer Components ׀ The NanoMolar Phosphate Assay Kit is for measurement of sub-micromolar (< 1 µM) concentrations of phosphate. The assay is based on reduction of the fluorescence intensity of the kit reagent by phosphate. It can be used for measurement of ATPase or GTPase activity of protein samples. It is suitable for high throughput screen (HTS) of drug targets with ATPase or GTPase activity. Since the assay is ultra-sensitive to phosphate, it is particularly useful to detect ATPase or GTPase activity where a limited level of phosphate is produced. The ATP or GTP concentration used for the assay should be as low as possible because ATP and GTP products contain some phosphate level. The ATP or GTP concentration should be below 50 µM. This product includes 100 ml of Reagent P1 and 1 ml of 100 x Reagent P2 and 0.1 ml of 1 mM potassium phosphate (KH2PO4). It is for 1000 assays using 96-well plates (100 ul of sample volume) or 4000 assays using 384-well plates (25 µl of sample volume). The following protocol is for assays using 96-well plates. Cuvettes may also be used for the measurement. |
| Order #: | PFD-NPA1000 |
| Unit Size: | 1000 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Concentration Measurement |
| More information: | Go to webpage |
356.00 € *
*All prices are net in Euro and do not include applicable taxes, shipping & handling, or other charges (e.g., customs duties).
Delivery time approx. 8 - 10 working days
- Phone: +49 (0)89 3799666-6
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- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
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