Description: | Biomolecules ׀ Polyphosphate Assay ׀ Inorganic polyphosphate is a linear molecule composed of tens or hundreds of phosphate residues linked together. In bacteria, polyphosphate kinase (PPK) converts polyphosphate and ADP to ATP. The MicroMolar Polyphosphate Assay Kit is for measurement of micromolar concentrations of polyphosphate. The assay is based on increase of the fluorescence intensity (emission 550 nm, excitation 415 nm) of the kit fluorescence dye PPD upon binding to polyphosphate. The assay is compatible with regular buffers and various phosphate compounds including inorganic phosphate, pyrophosphate, ATP, ADP and AMP. The assay kit can be used for measurements of polyphosphate in biological samples or environmental water samples. This product includes 300 ul of 100 x PPD dye and 30 ul of 1 mM sodium polyphosphate ( a 45-mer). It is for 1000 assays using 384-well plates (30 ul of sample volume) or 500 samples using 96-well plates (60 l of sample volume). Cuvettes may also be used for measurements. |
Order #: | PFD-PPD1000 |
Unit Size: | 1000 assays |
Supplier: | ProFoldin |
Shipping: | Blue Ice (-20°C) |
Storage: | See Manual |
Subcategory: | Concentration Measurement |
More information: | Go to webpage |
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