E. coli Biotin Protein Ligase Assay Kit Plus-500 (ProFoldin Product Code: BPL500KE)
| Description: | Antibacterial Drug Target ׀ Biotin protein ligase (BPL or BirA) is responsible for biotinylation of biotin-dependent proteins. It is an essential enzyme and an attractive target for anti-bacterial drug discovery. The Bacterial Biotin Protein Ligase Assay is based on measurement of the pyrophosphate generated from the BirA reaction using hydroxylamine in the place of protein as a biotin receptor. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of Bacterial BirA in drug discovery research. It may also be used for characterization of Bacterial BirA. This product includes the reagents for 500 assays in a 384-well plate assay format including 2 ml of 10 x Buffer, 0.170 ml of 100 x Biotin, 0.170 ml of 100 x hydroxylamine, 0.170 ml of 100 x ATP, 0.170 ml of 100 x E. coli BirA (5000 nM), 0.170 ml of 100 x pyrophosphatase (PPase, 10 U/ml) and 25 ml of Dye for phosphate detection. |
| Order #: | PFD-BPL500KE |
| Unit Size: | 500 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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