E. coli D-Alanine: D-Alanine Ligase Assay Kit Plus-100 (ProFoldin Product Code: DDA100KE)
| Description: | Antibacterial Drug Target ׀ D-Alanine-D-Alanine is one of the building blocks in peptidoglycan biosynthesis in bacteria. This dipeptide is generated by ligation between two D-Alanine molecules catalyzed by D-Alanine : D-Alanine ligase. The ligation reaction is coupled to the hydrolysis of ATP forming ADP and inorganic phosphate. The E. coli D-Alanine : D-Alanine Ligase Assay is based on measurement of the inorganic phosphate generated from the D-Alanine : D-Alanine ligation reaction. The inorganic phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli D-Alanine : D-Alanine ligase in drug discovery research. It may also be used for characterization of E.coli D-Alanine : D-Alanine ligase. This product includes the reagents for100 assays in a 384-well plate assay format including 0.400 ml of 10 x Buffer, 0.035 ml of 100 x Enzyme Substrate, 0.035 ml of 100 x E. coli D-Ala:D-Ala ligase (200 nM) and 5 ml of Dye for phosphate detection. |
| Order #: | PFD-DDA100KE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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