E. coli DNA Topoisomerase IV Assay Kit Plus-100 (enzyme included) (ProFoldin Product Code: DDC100KE)
| Description: | Antibacterial Drug Target ׀ DNA topoisomerase IV – DNA decatenation assay (spin-column format). DNA topoisomerase IV (the parC-parE complex) converts the concatenated DNA into decatenated DNA. It also relaxes supercoiled DNA. The DNA Topoisomerase IV Decatenation Assay is based the principle that the decatenated DNA is separated from the concatenated DNA by a spin-column or filtration process. The concatenated DNA stays on the column or filter, while the decatenated DNA is eluted. The eluted DNA is quantified by fluorescence. A high throughput assay based on the DNA relaxation activity is also available. This product includes the 600 ul of assay buffer (Buffer T4), 520 ul of 10 x concatenated DNA, 120 ul of 50 x ATP (10 mM), 550 ul of 0.4 M EDTA, 780 ul of 20 x fluorescence dye, 100 spin columns and 55ul of 100 x E.coli topoisomerase IV (500 nM) for 100 E. coli topoisomerase IV DNA decatenation assays. |
| Order #: | PFD-DDC100KE |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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