P. aeruginosa DNA Helicase ATPase assay Kit Plus-100 (ProFoldin Product Code: DNAB100KP)
| Description: | Antibacterial Drug Target ׀ DNA helicase (DnaB) hydrolyzes ATP as the source of molecular energy to carry out DNA unwinding required by the DNA replication process. Inhibition of the ATPase activity of DNA helicase blocks its DNA unwinding function. The DNA helicase ATPase assay can be used for high-throughput screen of DNA helicase inhibitors in drug discovery. The DNA Helicase ATPase Assay Kit is based on detection of the phosphate produced by the ATP hydrolysis reaction in the presence of DNA. The assay is in a 384-well plate format and the phosphate is detected using light absorbance at 650 nm. This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format. |
| Order #: | PFD-DNAB100KP |
| Unit Size: | 100 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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