DNA Topoisomerase I Assay Kit (enzyme not included) (ProFoldin Product Code: DRA020K)
| Description: | Antibacterial Drug Target ׀ DNA topoisomerases such as bacterial topoisomerase I convert supercoiled circular DNA into relaxed DNA (DNA relaxation reaction). The DNA Topoisomerase I Assay Kit is based on the principle that the relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA when the DNA interacts with fluorescence dye H19 (a fluorescence dye for DNA relaxation / supercoiling assay) in the presence of magnesium. When the supercoiled DNA is converted into its relaxed form, the fluorescent signal decreases. This product includes the reaction buffer (T1 Buffer), supercoiled plasmid DNA and fluorescence dye H19 for assaying 20 DNA relaxation reactions in a 96-well plate assay format or 40 assays in a 384-well assay format. The reaction buffer is optimized for bacterial topoisomerase I. |
| Order #: | PFD-DRA020K |
| Unit Size: | 20 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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