E. coli DNA Topoisomerase I Assay Kit Plus (E. coli Topo I included) (ProFoldin Product Code: DRA020KE)
| Description: | Antibacterial Drug Target ׀ DNA relaxation is an essential process during DNA replication. The primary function of topoisomerase I is DNA relaxation in both bacteria and mammalian cells. Bacterial topoisomerase IV and human topoisomerase II also have DNA relaxation activities. The Topoisomerase DNA relaxation Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA. Therefore, when the supercoiled DNA is converted into its relaxed form, the fluorescent signal decreases. The change of fluorescence intensity is used to measure the relaxation reaction. This product includes 160 ul of 10 x Buffer T1, 84 ul of 10 x supercoiled plasmid DNA, 10 ul of 100 x E. coli topoisomerase I, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format. |
| Order #: | PFD-DRA020KE |
| Unit Size: | 20 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
Add to request list
- Phone: +49 (0)89 3799666-6
- E-mail: info@biozol.de
MoBiTec is now part of BIOZOL
All orders and requests placed via the online shop on www.mobitec.com are processed by BIOZOL Diagnostica Vertrieb GmbH.
You will receive an order confirmation by fax or e-mail. More information regarding the ordering process can be found here.
Technical Service - Product Information
Viewed