S. aureus Gyrase DNA Supercoiling Assay Kit Plus (enzyme included) (ProFoldin Product Code: DSA020KSE)
| Description: | Antibacterial Drug Target ׀ DNA topoisomerases such as bacterial topoisomerase II (gyrase) convert relaxed circular DNA into supercoiled DNA. The DNA Topoisomerase II (Gyrase) Assay Kit is based on the principle that the supercoiled DNA and relaxed DNA yield different fluorescent intensity when interact with fluorescence dye H19. The relaxed DNA suppresses the fluorescent intensity much more than the supercoiled DNA. Therefore, when the relaxed DNA is converted into its supercoiled form, the fluorescent signal increases. The change of fluorescence intensity is used to measure the supercoiling reaction. This product includes160 ul of 10 x Buffer T2, 84 ul of 10 x relaxed DNA, 6 ul of 1500 x H19 dye, 90 ul of 10 x ATP, 10 ul 7.5 uM S. aureus gyrase, 180 ul of 2 M potassium glutamate and 550 ul of 10 x H19 dilution buffer. It is for 20 assays in a 96-well plate format. |
| Order #: | PFD-DSA020KSE |
| Unit Size: | 20 assays |
| Supplier: | ProFoldin |
| Shipping: | Blue Ice (-20°C) |
| Storage: | See Manual |
| Subcategory: | Drug Discovery |
| More information: | Go to webpage |
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